Urokinase-type Plasminogen Actvator (uPA) is a glycosylated serine protease that is synthesized in endothelial and kidney epithelial cells. There are two forms of uPA, the low molecular weight (LMW ~31 kDa) and high molecular weight (HMW ~55 kDa). The HMW form undergoes autoproteolysis resulting in the LMW plus and 18.5 kDa amino terminal fragment (ATF). It is this ATF that has been shown to inhibit proliferation and invasion of cancer cells by binding to uPA receptors (1).
This is an ELISA for the quantitative analysis of active uPA levels in biological fluid. This test kit operates on the basis of sandwich ELISA where free active uPA enzyme complexes with PAI-1 and is quantitated with the use of an HRP labeled secondary antibody.Provided is biotinylated PAI-1 bound to avidin coated wells. This allows active tPA present in the standard or unknown, to complex with PAI-1. Inactive or complexed uPA is removed in a subsequent wash step. A primary antibody specific for uPA is then added to each well followed by the HRP conjugated secondary antibody. The bound conjugated secondary antibody is detected by the addition of substrate, which generates an optimal color after 10 minutes. Quantitative test results may be obtained by the measure and comparison of the sample and standard absorbance readings when read with a microplate reader at 450 nm.
Oxford Biomedical Research
OBR公司开发,生产和销售检测试剂盒,重组蛋白和应用于生物医学和制药研究的抗体,现已具有400多种产品。
认识到减少疾病和改善人类健康将需要开发出更好的方法来识别和量化疾病的潜在危险因素,牛津生物医学研究(OBR)致力于为生物医学研究人员提供最佳的工具,用于分析氧化应激慢性炎症-两种最重要的危险因素,在多种人类疾病的发展中发挥关键作用,包括癌症,心血管疾病,糖尿病和神经变性。
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1 | 牛津生物医学研究 | IS20 | 1.0毫克 | |
2 | 牛津生物医学研究 | FR18 | 人血浆乳铁蛋白测定试剂盒 | 1套 |
3 | 牛津生物医学研究 | TA02 | 总抗氧化剂电源套件 | 1套 |
4 | 牛津生物医学研究 | PR32 | 抗兔CYP450 2E1 | 0.1毫升 |
5 | 牛津生物医学研究 | CE2B6.250 | 250毫克 | |
6 | 牛津生物医学研究 | GS70 | 0.1毫克 | |
7 | 牛津生物医学研究 | GS22 | 0.005毫克 | |
8 | 牛津生物医学研究 | PH91 | 100微克 | |
9 | 牛津生物医学研究 | PK50 | 面板 | |
10 | 牛津生物医学研究 | NS25 | 0.1毫克 | |
11 | 牛津生物医学研究 | UP33 | 0.05毫克 | |
12 | 牛津生物医学研究 | PK42 | 0.1毫克 | |
13 | 牛津生物医学研究 | PI66 | 1.0毫克 | |
14 | 牛津生物医学研究 | FS35 | 1套 | |
15 | 牛津生物医学研究 | MP12 | 0.001毫克 |