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℡ 4000-520-616
℡ 4000-520-616
Oxford Biomedical Research/Catalase Assay Kit/Kit/FR20
产品编号:FR20
市  场 价:¥14000.00
场      地:美国(厂家直采)
产品分类: 其它>配件>计数测量>
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电话号码:4000-520-616
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美  元  价:$700.00
品      牌: Oxford
公      司:OxfordBiomedicalResearch
公司分类:
Oxford Biomedical Research/Catalase Assay Kit/Kit/FR20
商品介绍
Product Overview

Background

Catalase is present in the peroxisomes of nearly all aerobic cells and serves to protect the cell from the toxic effects of hydrogen peroxide by catalyzing the decomposition of H2O2 (1,2). The mechanism of catalysis is not fully elucidated, but the overall reaction is as follows:2H2O2 —> 2H20 + O2Catalase has one of the highest catalytic activities reported, near the diffusion-controlled limit. Catalases are tetramers of four identical subunits (220 to 350 kD), each with a heme prosthetic group at the catalytic center. Eukaryotic catalases bind NADPH, which helps to stabilize the enzyme (3).In humans, the highest levels of catalase are found in the liver, kidney, and erythrocytes, where it is believed to account for the majority of hydrogen peroxide decomposition. Studies in a mouse model system have demonstrated the importance of catalase in preventing methemoglobin formation in erythrocytes (4). Catalase activity can be directly monitored in the ultraviolet region (7), however the UV assay is subject to interference due to absorption by protein and other components in biological samples.

 

Assay Principle

This colorimetric, cuvette based catalase assay involves two steps. Since the rate of dismutation of hydrogen peroxide to water and oxygen is proportional to the concentration of catalase, samples are first incubated with a known amount of hydrogen peroxide. The remaining hydrogen peroxide, following a fixed incubation period, is then determined by the oxidative coupling reaction of 4-aminophenazone (4-aminoantipyrene, AAP) and 3,5-dichloro-2-hydroxy-benzenesulfonic acid (DHBS) in the presence of H2O2 and catalyzed by horseradish peroxidase (6). The resulting quinoneimine dye is measured at 520nm.Notes: Catalase is very unstable at high dilution and should be kept cold and assayed within 30-60 minutes after dilution. Whole blood samples may be stored at 4°C for up to two weeks but it is recommended that samples be stored at –70°C for long-term storage. Red blood cell lysates, undiluted, are stable for 5 days at 4°C. Long term storage should be at –70°C. References1. Deisseroth, A. & Dounce, A.L., Physiol. Rev. 50, 319-375 (1970).2. Zamocky, M. & Koller, F. Prog. Biophys. Mol. Biol. 72, 19-66 (1999).3. Kirkman, H.N., et al., , J. Biol. Chem. 262, 660-666 (1987).4. Wakimoto, M., et al., Acta Med. Okayama 52, 233-237 (1998).5. Aebi, H. Methods Enzymol 105, 121-126 (1984).6. Fossati, P., et.al. Clin. Chem. 26, 227-231 (1980).

 

Spec Sheet
FR20.pdf
品牌介绍

Oxford Biomedical Research


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认识到减少疾病和改善人类健康将需要开发出更好的方法来识别和量化疾病的潜在危险因素,牛津生物医学研究(OBR)致力于为生物医学研究人员提供最佳的工具,用于分析氧化应激慢性炎症-两种最重要的危险因素,在多种人类疾病的发展中发挥关键作用,包括癌症,心血管疾病,糖尿病和神经变性。 


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